NURSING ANM AND GNM

Detailed explanation-1: -T-Streaking: In this method, the petri dish is divided into three quadrants by drawing the letter ‘T’. Similar to four-quadrant streaking, it is a discontinuous method where each quadrant is streaked after loop sterilisation, and the quadrant that is streaked last gives isolated colonies.

Detailed explanation-2: -The three sector streak or commonly referred to as the “T-Streak” is a basic, yet crucial skill used by microbiologist on a daily basis to isolate, identify, and study colonies of bacteria.

Detailed explanation-3: -The streaking is done using a sterile tool, such as a cotton swab or commonly an inoculation loop. This is dipped in an inoculum such as a broth or patient specimen containing many species of bacteria.

Detailed explanation-4: -The purpose of the streak plate is to obtain isolated colonies from an inoculum by creating areas of increasing dilution on a single plate. Isolated colonies represent a clone of cells, being derived from a single precursor cell.