NURSING ANM AND GNM

Detailed explanation-1: -Figure 1: Methylene blue is a simple and direct stain used for determining bacterial morphology (shape and arrangement). It is a cationic dye (positive charge) which stains the cell a blue color. The presence of negatively charged molecules in the cell (like DNA & RNA) causes the cell to stain blue.

Detailed explanation-2: -The Gram stain is especially useful as one of the first steps in the identification of a bacterial species, since it reveals both the morphology and the Gram reaction of the bacteria.

Detailed explanation-3: -Simple staining involves directly staining the bacterial cell with a positively charged dye in order to see bacterial detail, in contrast to negative staining where the bacteria remain unstained against a dark background.

Detailed explanation-4: -Simple staining allows one to observe the morphology and arrangement of the bacterial cells. Morphology refers to “form” or shape. We can use terms like spiral, bacilli (rod shaped), and cocci (spherical) to describe cell shapes.

Detailed explanation-5: -Simple staining can be of two types: simple positive staining and simple negative staining. In simple positive staining, basic dyes such as methylene blue or crystal violet are used. The basic dye gets attracted to the negatively charged genetic material of the microbial cytoplasm.