NURSING ANM AND GNM

Detailed explanation-1: -Flaming the inoculating loop sterilizes it and allows for the dilution of the inoculum. This will allow fewer organisms to be streaked in each area which will result in the desired isolation or separation of colonies.

Detailed explanation-2: -Don’t forget to sterilize. Flame the loop or use a new disposable loop after you streak each quadrant. Using a new or sterilized loop allows you to effectively dilute the inoculum on the plate and obtain isolated colonies by spreading the inoculum thinner and more evenly.

Detailed explanation-3: -By separating the plate into 4 sections and taking one loop from each section to try to make isolated colonies & sterilizing the loop in between each section.

Detailed explanation-4: -Flame the inoculating loop until it is red hot and then allow it to cool. 2. Remove a small amount of bacterial growth (either a loopful from a broth culture or a single colony from a plate or slant) with the sterile inoculating loop.