NURSING ANM AND GNM

Detailed explanation-1: -By separating the source of the amplicons’ (e.g., post-PCR) activities from the pre-PCR activities, the potential for contamination is significantly reduced. This separation is best illustrated by separating the facilities in space, so that there are two rooms where these activities occur (Fig. 2).

Detailed explanation-2: -One of the most common ways to monitor for contamination is to use “no template controls” (NTCs). In a standard 96-well plate qPCR setup, NTC wells contain all the qPCR reaction components components such as primers, reagents etc., with the exception of the DNA template [2].