NURSING EXAM QUESTIONS
MICROBIOLOGY
Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Magnesium oxide
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Silica gel
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Activated alumina
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Potassium permanganate
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Detailed explanation-1: -5. Which of the following cannot be used as adsorbent in Column adsorption chromatography? Explanation: The given options are all examples of adsorbents in Column adsorption except potassium permanganate.
Detailed explanation-2: -Pipette 0.5 ml from the mixture of potassium permanganate and potassium dichromate into the solvent layer above the silica gel in the packed column. Open the tap and continue filling the column with distilled water and elute it until the permanganate layer runs down the column.
Detailed explanation-3: -Paper chromatography is an example of partition chromatography. Thin layer chromatography and column chromatography are the examples of adsorption chromatography. Hence, the correct answer is option (a).
Detailed explanation-4: -The most common stationary phase for column chromatography is silica gel, followed by alumina.