NURSING EXAM QUESTIONS
PATHOLOGY AND GENETICS
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Single Fluorescent Immunoassay
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Single-Molecule ELISA
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Single Chemiluminescent ELISA
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Single Radioimmunoassay
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Detailed explanation-1: -A laboratory technique that uses antibodies linked to enzymes to detect and measure the amount of a substance in a solution, such as serum. The test is done using a solid surface to which the antibodies and other molecules stick.
Detailed explanation-2: -Principle of ELISA ELISA works on the principle that specific antibodies bind the target antigen and detect the presence and quantity of antigens binding. In order to increase the sensitivity and precision of the assay, the plate must be coated with antibodies with high affinity.
Detailed explanation-3: -Enzyme-linked immunosorbent assay (ELISA) is a method of target antigen (or antibody) capture in samples using a specific antibody (or antigen), and of target molecule detection/quantitation using an enzyme reaction with its substrate.
Detailed explanation-4: -Uses: It is used to detect the presence of antibodies, antigens, and other proteins and glycoproteins in the sample.