PATHOLOGY

Detailed explanation-1: -MPF is a dimer consisting of cyclin B and the Cdc2 protein kinase. A variety of further studies have confirmed this role of cyclin B, as well as demonstrating the regulation of MPF by phosphorylation and dephosphorylation of Cdc2 (Figure 14.16). In mammalian cells, cyclin B synthesis begins in S phase.

Detailed explanation-2: -This suggested the key role of this molecule in the induction of the M-phase of cell cycle in all eukaryotic cells. After numerous efforts, MPF was identified as a complex of Cyclin-Dependent Kinase 1 (CDK1) and its regulatory subunit cyclin B [6–9].

Detailed explanation-3: -MPF is composed of two subunits: Cyclin-dependent kinase 1 (CDK1), the cyclin-dependent kinase subunit. It uses ATP to phosphorylate specific serine and threonine residues of target proteins. Cyclin, a regulatory subunit.

Detailed explanation-4: -The protein maturation promoting factor (MPF) is the cyclin and cyclin dependent kinase complex that regulates the transition between G2 and M phase.

Detailed explanation-5: -Abstract. Cyclin-dependent kinases (CDKs) are protein kinases characterized by needing a separate subunit-a cyclin-that provides domains essential for enzymatic activity. CDKs play important roles in the control of cell division and modulate transcription in response to several extra-and intracellular cues.