PATHOLOGY

PATHOLOGY MCQ

GENETICS AND DISEASE

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
Gel electrophoresis is used to create a unique DNA fingerprint composed of different-sized DNA fragments. What are the molecules responsible for cutting DNA into fragments of different sizes prior to running gel electrophoresis?
A
transferase enzymes
B
restriction enzymes
C
hydrolase enzymes
D
ligase enzymes
Explanation: 

Detailed explanation-1: -Gel electrophoresis is basically the process by which we take the DNA, and run an electric charge through it. The DNA, being negatively charged by default, will move towards the positive side. As this happens, he DNA with lower density will travel less distance up. This can create a very unique pattern of DNA.

Detailed explanation-2: -Gel electrophoresis is a technique used to separate DNA fragments according to their size. DNA samples are loaded into wells (indentations) at one end of a gel, and an electric current is applied to pull them through the gel. DNA fragments are negatively charged, so they move towards the positive electrode.

Detailed explanation-3: -How was the first DNA fingerprint produced? The first step of DNA fingerprinting was to extract DNA from a sample of human material, usually blood. Molecular ‘scissors’, called restriction enzymes?, were used to cut the DNA. This resulted in thousands of pieces of DNA with a variety of different lengths.

Detailed explanation-4: -First, we use the polymerase chain reaction (PCR) technique to copy a tiny fragment of DNA so that there is enough to use in gel electrophoresis. Gel electrophoresis uses gel and electricity to separate DNA fragments based on size, creating a distinct pattern that represents an individuals genetic information.

There is 1 question to complete.