PATHOLOGY

PATHOLOGY MCQ

GENETICS AND DISEASE

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
Gel electrophoresis separates DNA according to size. Which is true regarding movement of the DNA?
A
The longest pieces of DNA will move the least distance.
B
The longest pieces of DNA will move the greatest distance.
C
The shortest pieces of DNA line up along the middle.
D
The shortest pieces of DNA line up an equal distance from the middle
Explanation: 

Detailed explanation-1: -The DNA fragments that are shortest will travel farthest, while the longest fragments will remain closest to the origin. Using the same basic principles, electrophoresis can also be used to separate RNA and proteins.

Detailed explanation-2: -The correct statement is option a-Smaller fragments travel more quickly than larger fragments. Agarose gel electrophoresis is a molecular technique that is used to separate molecules based on the size. The gel used for separation is made up of agarose which is a solidifying agent.

Detailed explanation-3: -Gel electrophoresis is used to segregate DNA fragments according to mass and size. DNA is negatively charged and it will travel towards the positive electrode. Hence separation will be on the size of the fragments. The smaller DNA molecules move faster and farthest followed by the larger ones.

Detailed explanation-4: -In agarose gel electrophoresis, the DNA fragments separate out (resolve) according to their size or length because of the sieving property of agarose gel.

There is 1 question to complete.