PATHOLOGY MCQ
GENETICS AND DISEASE
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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pulled toward that end by gravity
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attracted to complementary DNA fragments at that end of the gel
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attracted to the positively charged end of the gel
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repelled by hydrophobic molecules at the other end of the gel
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Detailed explanation-1: -DNA is negatively charged, therefore, when an electric current is applied to the gel, DNA will migrate towards the positively charged electrode. Shorter strands of DNA move more quickly through the gel than longer strands resulting in the fragments being arranged in order of size.
Detailed explanation-2: -DNA fragments are negatively charged, so they move towards the positive electrode.
Detailed explanation-3: -Why do the fragments of DNA in gel electrophoresis move away from the negative cathode? DNA is negatively charged and attracted to the positive anode. DNA is positively charged and attracted to the positive anode.
Detailed explanation-4: -In gel electrophoresis, the positive pole is called the anode and the negative pole is called the cathode; therefore, the charged particles will migrate to the respective nodes.
Detailed explanation-5: -Agarose gel electrophoresis is used to resolve DNA fragments on the basis of their molecular weight. Smaller fragments migrate faster than larger ones; the distance migrated on the gel varies inversely with the logarithm of the molecular weight.