PATHOLOGY MCQ
GENETICS AND DISEASE
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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inserting the plasmid into a bacterium
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removing the plasmid from the bacterium
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cutting the plasmid with restriction enzymes
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inserting the gene of the parent generation into the plasmid
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Detailed explanation-1: -In a typical cloning experiment, researchers first insert a piece of DNA, such as a gene, into a circular piece of DNA called a plasmid. This step uses restriction enzymes and DNA ligase and is called a ligation. After a ligation, the next step is to transfer the DNA into bacteria in a process called transformation.
Detailed explanation-2: -Key steps in the process of bacterial transformation: (1) competent cell preparation, (2) transformation of cells, (3) cell recovery, and (4) cell plating.
Detailed explanation-3: -Steps of DNA cloning Cut open the plasmid and “paste” in the gene. This process relies on restriction enzymes (which cut DNA) and DNA ligase (which joins DNA). Insert the plasmid into bacteria.
Detailed explanation-4: -The Cloning Process. The DNA cloning process involves isolating the gene of interest and cutting it with restriction enzymes. The same enzymes are used to cut a vector, such as a bacterial plasmid. A bacterial plasmid is a small circular piece of DNA.