PATHOLOGY MCQ
GENETICS AND DISEASE
Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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PCR and gel electrophoresis
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Gene transfer and gel electrophoresis
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PCR and a Karyogram
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Gene transfer and PCR
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Detailed explanation-1: -First, we use the polymerase chain reaction (PCR) technique to copy a tiny fragment of DNA so that there is enough to use in gel electrophoresis. Gel electrophoresis uses gel and electricity to separate DNA fragments based on size, creating a distinct pattern that represents an individuals genetic information.
Detailed explanation-2: -While RFLP and PCR techniques analyze DNA extracted from the nucleus of a cell, mtDNA technology analyzes DNA found in a different part of the cell, the mitochondrion.
Detailed explanation-3: -The DNA testing process is comprised of four main steps, including extraction, quantitation, amplification, and capillary electrophoresis.
Detailed explanation-4: -Gel electrophoresis is a technique in which fragments of DNA are pulled through a gel matrix by an electric current, and it separates DNA fragments according to size. A standard, or DNA ladder, is typically included so that the size of the fragments in the PCR sample can be determined.