PATHOLOGY

Detailed explanation-1: -Kohler and Milstein [2] developed a system in which antibody-producing B cells were fused with immortal cancerous cell lines such as myeloma cells [3] (Fig. 1), creating an immortal hybrid cell line that produces antibodies limitlessly. Hybridoma technology produces monoclonal antibodies (mAbs) specific to antigens.

Detailed explanation-2: -Hybridoma is a culture of hybrid cells that results from the fusion of B cells and myeloma cells. Hybridoma technology produces hybridomas.

Detailed explanation-3: -Production of monoclonal antibodies requires immortalization of splenocytes by somatic fusion to a myeloma cell line partner (hybridomas).

Detailed explanation-4: -Formation of monoclonal antibodies The spleen cells are fused with human cancerous white blood cells called myeloma cells to form hybridoma cells which divide indefinitely. These hybridoma cells divide and produce millions of monoclonal antibodies specific to the original antigen.

Detailed explanation-5: -Hybridoma technology is a technology of formation of hybrid cell lines i.e., hybridomas by the fusion of B-lymphocyte cell and a myeloma cell.