IMMUNOGLOBULINS
MONOCLONAL ANTIBODIES
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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In the clonning of hybridoma cell line by limiting dilution the in vivo propagation is done with
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Mouse ascites method
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Grafting technology
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Cell immobilization
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Tissue culture method
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Explanation:
Detailed explanation-1: -Below are some considerations for immunizing mice for hybridoma generation. BALB/c splenocytes and parental myeloma cells of BALB/c mice are recommended for the following reasons: highly immune reactive, well-characterized, availability of myeloma cells from the same genetic strain.
Detailed explanation-2: -Hybridoma cell lines are widely used to create mAbs. This is achieved by immunizing a mouse with a target antigen, thereby eliciting an immune response. The B lymphocytes, taken from the immunized mouse spleen, produce anti-bodies to the antigen.
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