IMMUNOLOGY

Detailed explanation-1: -V Intraperitoneal Injection The injection site should be in the lower left or right quadrant of the abdomen because vital organs are absent from this area. Many operators recommend restraining the animal in a ‘head-down, ‘ or Trendelenburg, position, ensuring that the viscera will be displaced cranially.

Detailed explanation-2: -Intraperitoneal injection is a common technique that safely delivers a substance into the peritoneal cavity but can induce high stress in animals. The authors have developed a new method for administering intraperitoneal injections in mice, with the goal of causing less stress during handling and injection.