IMMUNOGLOBULINS
MONOCLONAL ANTIBODIES
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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In the preparation of myeloma cells they are cultured with ____
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MERCAPTOPURINE
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8 AZAGUANINE
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5 HYDROYGUANINE
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AMINOTRYPTINE
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Explanation:
Detailed explanation-1: -Myeloma cells are immortalized cells that are cultured with 8-azaguanine to ensure their sensitivity to the hypoxanthine-aminopterin-thymidine (HAT) selection medium used after cell fusion. A week before cell fusion, myeloma cells are grown in 8-azaguanine. Cells must have high viability and rapid growth.
Detailed explanation-2: -A typical monoclonal antibody production process Myeloma cells are prepared for fusion. Fusion-Myeloma cells and isolated splenocytes are fused together to form hybridomas in the presence of polyehthylene glycol(PEG), which causes cell membranes to fuse.
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