IMMUNOLOGY

Detailed explanation-1: -The monoclonal antibody you want to produce will have a specific antigen. This antigen is inserted into a mouse to initiate an immune response in the mouse. The mouse immune response occurs. The mouse’s immune response takes place and antibodies are created against the supposed pathogen.

Detailed explanation-2: -Spleen cells, which produce the lymphocytes, are removed from the mouse. The spleen cells are fused with human cancerous white blood cells called myeloma cells to form hybridoma cells which divide indefinitely. These hybridoma cells divide and produce millions of monoclonal antibodies specific to the original antigen.

Detailed explanation-3: -These transgenic mice have the potential to provide human immunoglobulins once vaccinated with a specific antigen. The B cells made by these transgenic mice are used to produce monoclonal antibodies.