IMMUNOGLOBULINS
MONOCLONAL ANTIBODIES
|
Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
|
Which of the following are correct regarding HAT medium
|
|
HAT medium is a selective medium
|
|
|
Aminopterin in the HAT medium blocks de-nova pathway of nucleotide synthesis
|
|
|
Salvage pathway requires aminopterin and thymidine
|
|
|
Hypoxanthine is converted to guanine by HGPRT enzyme
|
Explanation:
Detailed explanation-1: -HAT medium has been used to screen for hybridomas (q.v.) by mixing TK+ HGPRT− myeloma cells with antigen-stimulated TK− HGPRT+ spleen cells. The hybrid TK+ HGPRT+ clones that survive in HAT medium are then assayed for monoclonal antibodies specific to the immunizing antigen.
Detailed explanation-2: -Expert-Verified Answer. C) Myeloma cells are made deficient in the hypoxanthine-guanine phosphoribosyl transferase (HGPRT) enzyme.
Detailed explanation-3: -HAT medium definition. A selection medium for hybrid cell lines; contains hypoxanthine; aminopterin; thymidine. Only cell lines expressing both hypoxanthine phosphoribosyl transferase (HPRT+) and thymidine kinase (TK+) can survive in this medium.
There is 1 question to complete.