IMMUNOLOGY

Detailed explanation-1: -In addition to blocking, it is essential to perform thorough washes between each step of the ELISA. Washing steps are necessary to remove non-bound reagents and decrease background, thereby increasing the signal to noise ratio.

Detailed explanation-2: -Washing Washing is typically repeated 3-5 times between each step in the ELISA to thoroughly remove unbound material. Usually the wash solution is only briefly retained on the plate. Excess wash solution must be removed in the final wash step to prevent the dilution of the reagents added in the subsequent stage.