IMMUNOLOGY

Detailed explanation-1: -a Radial Immunodiffusion This method requires polyclonal antibody. The method is based on the precipitation in agarose gel of antigen-antibody complexes, and this does not occur with monoclonal antibodies as more than one binding site on the antigen is required for complex formation.

Detailed explanation-2: -Immunodiffusion testing is most often used to detect antibodies to fungal pathogens in dogs, such as Aspergillus fumigatus, 3 Coccidioides immitis, 4 and Blastomyces dermatitidis. It is also used to detect antibodies to Brucella canis.