MISCELLENOUS
VACCINATION
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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The antibody molecules are too large to diffuse through the agar.
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The precipitin lattice is too large to diffuse through the agar.
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Methanol, added once the arc forms, denatures the protein and blocks diffusion.
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The antigen molecules are chemically coupled to the gel matrix.
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Detailed explanation-1: -In the Ouchterlony assay, we see a sharp precipitin arc form between antigen and antiserum. Why does this arc remain visible for a long time? The antibody molecules are too large to diffuse through the agar. The precipitin lattice is too large to diffuse through the agar.
Detailed explanation-2: -The radial immunodiffusion assay is used to quantify antigen by measuring the size of a precipitation zone in a gel infused with antibodies.
Detailed explanation-3: -Among flocculation tests, complement-fixation tests are the most common. These are based on the precipitation, or flocculation, that takes place when an antibody and specially prepared antigens (substances that provoke antibody production in the body) are mixed together.