IMMUNOLOGY

Detailed explanation-1: -The rocket immunoelectrophoresis technique or electroimmunodiffusion (EID) (1) is a simple, fast, and reproducible technique for quantitation of a single protein, and is also applicable in a protein mixture. Several unknown samples can be analyzed on a single plate.

Detailed explanation-2: -It is called as “rocket electrophoresis” due to the appearance of the precipitin bands in the shape of cone-like structures (rocket appearance) at the end of the reaction. In rocket immunoelectrophoresis, antigen migrates in an electric field in a layer of agarose containing an appropriate antibody.

Detailed explanation-3: -The difference between them is the concentration of proteins that they can detect: while rocket immunoelectrophoresis can detect protein concentrations as low as 5 g/mL, radial immunodiffusion requires at least 50 g/mL of proteins or higher to be detected.