IMMUNOLOGY

Detailed explanation-1: -Leishman Stain Solution is used for staining blood smears to differentiate blood corpuscles, malarial parasites, trypanosomers, etc. It can be used as an alternative to Giemsa and for use in staining bone marrow sections. It serves the purpose of investigating sample material of human origin.

Detailed explanation-2: -Blood films are routinely stained with a Romanowsky-type stain (e.g., Wright or Wright-Giemsa) either manually or using an automatic slide stainer. Romanowsky-type stains are composed of a mixture of eosin and oxidized methylene blue (azure) dyes.

Detailed explanation-3: -Leishman stain, also known as Leishman’s stain, is a type of the Romanowsky stain, and is similar to Wright Stain. It is also known as eosin-polychrome methylene blue as it is composed of a methanolic mixture of “polychromed” methylene blue (i.e., demethylated into several azure colors) and eosin.