MICROANATOMY

Detailed explanation-1: -ELISA test uses antibodies that react to human blood to tell if mammal blood is from human. However in rare cases, it can be confused with chimpanzee and gorilla blood. “enzyme-linked immunosorbent assay.” ELISA is a test that detects and measures antibodies in your blood.

Detailed explanation-2: -ELISA stands for enzyme-linked immunoassay. It is a commonly used laboratory test to detect antibodies in the blood. An antibody is a protein produced by the body’s immune system when it detects harmful substances, called antigens.

Detailed explanation-3: -Competitive ELISA An unlabeled primary antibody is coated onto the wells of a 96 well microtiter plate. This primary antibody is then incubated with unlabeled standards and samples with unknown protein content. After this reaction is allowed to go into equilibrium, conjugated antigen or enzyme-linked antibody is added.

Detailed explanation-4: -Enzyme-linked immunosorbent assay (ELISA) ELISAs detect and amplify antigen–antibody reactions by using covalently bound enzyme–antibody molecules. The presence of the enzyme (indicating presence of the antigen) is detected by the addition of the appropriate substrate.

Detailed explanation-5: -Peroxidase enzyme is used which when reduced converts the non-fluorescent molecules into fluorescent molecules. This test is used in the treatment of a variety of diseases like HIV etc.