MICROANATOMY

Detailed explanation-1: -Confocal imaging: 0-1000 µm beneath specimen surface As the powerhouses of many life science research laboratories, confocal systems allow scientists to study life processes in real time as they occur.

Detailed explanation-2: -Specimens that have three-dimensional structure that is to be studied with the confocal microscope, have to be mounted in such a way as to preserve the structure. Some sort of spacer, such as fishing line or a piece of coverslip, is commonly placed between the slide and the coverslip to avoid deforming the specimen.

Detailed explanation-3: -Confocal microscopy is an optical method that can provide high-resolution images of fresh, non-fixed, thick pieces of tissue, which are optically sliced, instead of using a microtome blade.