INTRODUCTION TO MICROSCOPY CELL BIOLOGY
MICROSCOPY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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You must see the lens aligned with the stage.
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You must position the lens 90 degrees away
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You must hear a “click” while turning the lens.
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You must see the lens glowing.
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Detailed explanation-1: -How could you know that the objective lens that you will be using is in the proper position for focusing? You must see the lens aligned with the stage.
Detailed explanation-2: -Always start with a low power objective (4X or 10X) clicked into place.
Detailed explanation-3: -Start by rotating the objective lens to lowest power. Place a slide on the stage, label side up, with the coverslip centered. On LOW POWER ONLY, use the coarse focus knob to get the object into focus. If you cannot see anything, move the slide slightly while viewing and focusing.
Detailed explanation-4: -When a particular objective has been fully rotated into position, you will hear or feel a click as that objective locks into place. The objective lens is the second of the two lenses doing the actual magnifying in a compound microscope, so if it is not snapped into proper position, you won’t see the proper image.