MICROANATOMY

Detailed explanation-1: -Iris Diaphragm controls the amount of light reaching the specimen. It is located above the condenser and below the stage. Most high quality microscopes include an Abbe condenser with an iris diaphragm. Combined, they control both the focus and quantity of light applied to the specimen.

Detailed explanation-2: -Condenser, The condenser under the stage focuses the light on the specimen, adjusts the amount of light on the specimen, and shapes the cone of light entering the objective.

Detailed explanation-3: -Typically, the condenser focuses the image of the light source directly onto the plane of the specimen, a technique called critical illumination.