INTRODUCTION TO MICROSCOPY CELL BIOLOGY
MICROSCOPY
Question
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Here are the settings on a microscope:Ocular Lens:10X Scanning Lens:4X Low Power Lens:15X High Power Lens:50X You measure the field of view under scanning power as 4.8 mm. Calculate (in micrometers) the field of view that would appear on low power?
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1.28
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1280
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3.2
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3120
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Explanation:
Detailed explanation-1: -The same principle apply to stereo microscopes, a 10X eye piece combined with a 4X objective lens will produce 40X magnification. Some stereo microscopes equipped with continues zoom objective lens with magnification from 0.75X-7.5X. The total magnification will be 7.5X to 75X when combined with 10X ocular lens.
Detailed explanation-2: -A compound microscope has multiple lenses: the objective lens (typically 4x, 10x, 40x or 100x) is compounded (multiplied) by the eyepiece lens (typically 10x) to obtain a high magnification of 40x, 100x, 400x and 1000x. Higher magnification is achieved by using two lenses rather than just a single magnifying lens.
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