INTRODUCTION TO MICROSCOPY CELL BIOLOGY
MICROSCOPY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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In preparing temporary mounts for microscopic examination, why the specimen has to be thin?
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To make the specimen easier to be focused
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To make the specimen easier to be covered by the cover slip
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To reduce the amount of stain used
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To allow light pass through the specimen to the objective
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Explanation:
Detailed explanation-1: -That the specimen is thin and flat so that only a single layer of cells is present. That some components have been differentially coloured (stained) so that they can be clearly distinguished.
Detailed explanation-2: -In a wet mount, the specimen is suspended in a drop of liquid (usually water) located between slide and cover glass. The water refractive index of the water improves the image quality and also supports the specimen.
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