MICROANATOMY

Detailed explanation-1: -Excitation filters permit the passage of specific wavelengths of light (excitation wavelengths) on the way towards the specimen. Barrier/emission filters attenuate the excitation wavelengths and permit only the selected emission wavelengths to pass towards the eye or the detector.

Detailed explanation-2: -There are many fluorescent molecules called fluorophores or fluorochromes such as fluorescein, Alexa Fluors, or DyLight 488, which can be chemically linked to a different molecule which binds the target of interest within the sample.

Detailed explanation-3: -The most common light source for fluorescence microscopy, chosen for its high brightness (known technically as luminance or radiance) in the ultraviolet and visible spectrum, is the mercury arc lamp.