MICROANATOMY

INTRODUCTION TO MICROSCOPY CELL BIOLOGY

MICROSCOPY

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
Which component of fluorescence microscope allows only light falling in absorption range of florophore to enter sample
A
Emission Filter
B
Barrier filter
C
Dichroic mirro
D
Excitation Filter
Explanation: 

Detailed explanation-1: -Excitation filters permit the passage of specific wavelengths of light (excitation wavelengths) on the way towards the specimen. Barrier/emission filters attenuate the excitation wavelengths and permit only the selected emission wavelengths to pass towards the eye or the detector.

Detailed explanation-2: -There are many fluorescent molecules called fluorophores or fluorochromes such as fluorescein, Alexa Fluors, or DyLight 488, which can be chemically linked to a different molecule which binds the target of interest within the sample.

Detailed explanation-3: -The most common light source for fluorescence microscopy, chosen for its high brightness (known technically as luminance or radiance) in the ultraviolet and visible spectrum, is the mercury arc lamp.

There is 1 question to complete.