INTRODUCTION TO MICROSCOPY CELL BIOLOGY
MICROSCOPY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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It has a smaller field of view and isn’t necessary
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Moving the stage up you may cause the lens to hit the slide
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You are allowed to use the coarse adjustment knob on high power
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None of the above
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Detailed explanation-1: -CAUTION: NEVER, EVER, use the coarse adjustment knob with the high power objective. This could break the lens. Multiply the power of the ocular lens, by the power of the objective lens.
Detailed explanation-2: -Coarse Adjustment Knob-The coarse adjustment knob located on the arm of the microscope moves the stage up and down to bring the specimen into focus. The gearing mechanism of the adjustment produces a large vertical movement of the stage with only a partial revolution of the knob.
Detailed explanation-3: -Why should you never use the coarse adjustment knob on high power? The coarse adjustment knob should not be used on high power because it will crack the slide. When do you use the fine adjustment knob? The fine adjustment knob is used to focus on the specimen when using the high power objective.