PATHOLOGY MCQ
GENETICS AND DISEASE
Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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dissolving blue dye
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restriction enzymes
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surgically cutting DNA under microscope
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None of the above
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Detailed explanation-1: -Restriction endonucleases are enzymes that cleave DNA molecules into smaller fragments.
Detailed explanation-2: -Restriction enzymes, also called restriction endonucleases, recognize a specific sequence of nucleotides in double stranded DNA and cut the DNA at a specific location. They are indispensable to the isolation of genes and the construction of cloned DNA molecules.
Detailed explanation-3: -Restriction endonuclease enzymes cut the DNA strands from within and at specific sites. These sites are known as recognition sequences. Every restriction endonuclease has a specific sequence and cuts the DNA wherever the sequence is encountered.
Detailed explanation-4: -Restriction enzymes are DNA-cutting enzymes. Each enzyme recognizes one or a few target sequences and cuts DNA at or near those sequences. Many restriction enzymes make staggered cuts, producing ends with single-stranded DNA overhangs. However, some produce blunt ends.