IMMUNOLOGY

Detailed explanation-1: -Basic ELISA principles ELISA (which stands for enzyme-linked immunosorbent assay) is a technique to detect the presence of antigens in biological samples. An ELISA, like other types of immunoassays, relies on antibodies to detect a target antigen using highly specific antibody-antigen interactions.

Detailed explanation-2: -In an ELISA, an antibody, known as a primary antibody, attaches to a target protein, which is immobilized on a solid surface, such as a well in a plate. A secondary antibody binds to this antibody-target protein complex, aiding in the detection of the target protein.

Detailed explanation-3: -Step 1: Capture antibody binds to ELISA plate wells. Step 2: Add sample to well – antigen within the sample binds to the capture antibody. Step 3: Wash microplate – Unbound material is washed away, leaving only the antigen of interest. More items