IMMUNOGLOBULINS
MONOCLONAL ANTIBODIES
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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hypoxanthine aminopterin thymidine
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Hydroxy acetic tetrahydrate
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Hypoxy amiopterin thymidine
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None of these
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Detailed explanation-1: -Hybridoma selection after fusion of myelomas and spleen cells is a critical step in monoclonal antibody production. Often scientists use the HAT (hypoxanthine-aminopterin-thymidine) method to accomplish this task.
Detailed explanation-2: -Complete answer: HAT(hypoxanthine–aminopterin–thymidine) medium is used for the selection of fused hybridoma cells in the process of monoclonal antibody production. After fusion of myelomas and spleen cells, HAT medium is used.
Detailed explanation-3: -HAT medium contains hypoxanthine-aminopterin-thymidine; aminopterin blocks the ability of cells to synthesize by de novo synthesis while hypoxanthine and thymidine is present to be used by cells with HGPRT to survive using salvage pathways for nucleotide synthesis.