IMMUNOLOGY

Detailed explanation-1: -The humanization process takes advantage of the fact that production of monoclonal antibodies can be accomplished using recombinant DNA to create constructs capable of expression in mammalian cell culture.

Detailed explanation-2: -Recombinant monoclonal antibodies are antibodies that are produced using recombinant DNA technology. Conventional monoclonal antibodies are generated by immunizing animals with immunogens to provoke immune responses.

Detailed explanation-3: -Chimeric and Humanized Antibodies Chimeric antibodies are molecules made up of domains from different species. For example, the Fc region or all the constant regions of a mouse mAb may be replaced with those of a human or (any other species) antibody.

Detailed explanation-4: -The genes of the B cell lineage present in the hybridoma cells produce a specific antibody with a specific epitope; this antibody is known as “monoclonal antibody.” There may be other hybridomas present in other wells producing antibodies specific to another epitope for the same antigen.