IMMUNOLOGY

Detailed explanation-1: -Transmission electron microscopy (TEM) is the only imaging technique allowing the direct visualization of viruses, due to its nanometer‐scale resolution.

Detailed explanation-2: -The electron microscope uses a beam of electrons and their wave-like characteristics to magnify an object’s image, unlike the optical microscope that uses visible light to magnify images.

Detailed explanation-3: -To prepare a virus sample for a negative stain transmission electron microscopy, a virus suspension is applied to a TEM grid specimen support, which is a 3 mm diameter fragile specimen screen coated with a few nanometers of plastic film.

Detailed explanation-4: -Electron Microscopy It does this by firing electrons at a sample and seeing how they interact with it. A computer then interprets this information to produce an image. This enables us to visually investigate different stages of virus infection inside cells.

Detailed explanation-5: -Most viruses are small enough to be at the limit of resolution of even the best light microscopes, and can be visualized in liquid samples or infected cells only by EM (electron microscopy).