RESEARCH METHODOLOGY

Detailed explanation-1: -One thing that might affect indoor microbes is exposure to light. Light can cause chemical reactions within microbes that harm their DNA or the proteins they need to survive and function.

Detailed explanation-2: -In general, the higher the temperature, the more easily microorganisms can grow up to a certain point. Very high and low temperatures both obstruct the enzyme processes microorganisms depend on to survive.

Detailed explanation-3: -Traditionally, the growth curve measurements are performed by measuring the OD of the bacteria, which is related to the cell number, in cuvettes at the wavelength of 600 nm using photometry at desired time points with intervals of 30–60 min [3, 4].

Detailed explanation-4: -It has been determined that in a closed system or batch culture (no food added, no wastes removed) bacteria will grow in a predictable pattern, resulting in a growth curve composed of four distinct phases of growth: the lag phase, the exponential or log phase, the stationary phase, and the death or decline phase.