IMMUNOGLOBULINS
COMPLEMENT SYSTEM
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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They link to the proteins used
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The catalyze reactions
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They provide a colormetirc indication of antigen exposure
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They are used to detect DNA
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Detailed explanation-1: -The primary antibody detects the antigen in the specimen, but the secondary antibody can be designed to have a fluorophore or enzyme complex attached to it for the purposes of visualization.
Detailed explanation-2: -The secondary antibody is conjugated with an enzyme because it shows the concentration, The enzyme substrate reaction shows a color change, which again shows how many antibodies are present. Disease samples were collected from two patients and subjected to serial dilutions before running an ELISA.
Detailed explanation-3: -Why are enzymes used in this immunoassay? Enzymes provide a way to see whether the primary antibody has attached to its target (antigen) in the microplate well. Primary and secondary antibodies are invisible, so a detection method is necessary. The enzyme HRP is linked to the second antibody.