MRCP UK EXAMINATIONS

Detailed explanation-1: -The Genexpert diagnoses TB by detecting the presence of TB bacteria. The test is a molecular TB test which detects the DNA in TB bacteria. It uses a sputum sample and can give a result in less than 2 hours. The Genexpert can also detect the genetic mutations associated with resistance to the drug Rifampicin.

Detailed explanation-2: -GeneXpert is found to be a rapid test for the diagnosis of TB and rifampicin resistance when compared to smear microscopy and culture. A smear-positive and GeneXpert negative result gives a high suspicion of NTM infection.

Detailed explanation-3: -The cartridge is loaded into the GeneXpert Analyzer, which subsequently detects the presence of TB DNA and any mutations that confer rifampin resistance. Internal controls are present within each cartridge to assess the presence of inhibitors that may cause false negative results.

Detailed explanation-4: -[7] Advantage of RT-PCR over GeneXpert is the ability to process larger number of samples simultaneously. So, The RT-PCR is best suitable for mass scale testing and use of GeneXpert can be lifesaving in emergency settings.