GENERAL HISTOLOGY

Detailed explanation-1: -Ammoniacal silver stains are most commonly used for reticular fiber and basement membrane staining. A variety of methods are available including Snook’s, Wilder’s, Foot’s, Jones’s, and Gomori’s periodic acid-methenamine silver method (PAMS).

Detailed explanation-2: -Abstract. Introduction: Silver stain of reticular fibres demonstrates the fine structure of the cardiac collagen network. However, nuclei are also stained with current techniques, a drawback that makes computer image analysis difficult.

Detailed explanation-3: -Reticulin stain uses silver impregnation to detect reticulin fibers, which are made of type 3 collagen. The fibers appear black against a gray to light pink background. In the liver, such fibers are present as part of the extracellular matrix in the space of Disse.

Detailed explanation-4: -Reticular fibers are composed of collagen and form a delicate framework around nerve fibers, fat cells, lymph nodes, and smooth and skeletal muscle fibers. They are also not visualized by an H&E.