GENERAL HISTOLOGY

Detailed explanation-1: -Cells are pushed into stratum granulosum and become flattened and diamond shaped. The cells accumulate keratohyalin granules mixed between tonofibrils. Cells continue to stratum corneum where they flatten and lose organelles and nuclei. The keratohyalin granules turn tonofibrils into a homogenous keratin matrix.

Detailed explanation-2: -Keratinization begins in the stratum spinosum, although the actual keratinocytes begin in the stratum basale. They have large pale-staining nuclei as they are active in synthesizing fibrilar proteins, known as cytokeratin, which build up within the cells aggregating together forming tonofibrils.

Detailed explanation-3: -The cells of the stratum granulosum (SGR) accumlate dense basophilic keratohyalin granules (seen on the close-up view). These granules contain lipids, which along with the desmosomal connections, help to form a waterproof barrier that functions to prevent fluid loss from the body.