GENERAL HISTOLOGY

Detailed explanation-1: -The ultracentrifuge is also used to separate cellular components on the basis of their buoyant density, independently of their size and shape. In this case the sample is usually sedimented through a steep density gradient that contains a very high concentration of sucrose or cesium chloride.

Detailed explanation-2: -The density of the gradient medium must be greater than the density of the particles to be separated. By this method, the particles will never sediment to the bottom of the tube, no matter how long the centrifugation time (Figure 3).

Detailed explanation-3: -In biochemistry and cell biology, differential centrifugation (also known as differential velocity centrifugation) is a common procedure used to separate organelles and other sub-cellular particles based on their sedimentation rate.

Detailed explanation-4: -The most important and most dense particle sediments are first followed by less dense and smaller particles. This tells us that the cellular organelle targeting the highest of the centrifuged mixture is going to be the mitochondria thanks to its less density and little size.

Detailed explanation-5: -The ultracentrifuge uses centrifugal force to separate larger molecules from smaller ones due to their different densities. it is the same as normal centrifugation, but the speeds are much higher. Centrifugation achieves this separation through a pressure gradient generated by fast spinning and gravity.