GENERAL HISTOLOGY

INTRODUCTION

MICROSCOPE PARTS MAGNIFICATION RESOLUTION

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
Before you move from low power to medium power or medium power to high power what should you ALWAYS do?
A
turn the coarse adjustment knob
B
change the setting on the diaphragm
C
turn the fine adjustment knob
D
center the specimen in the field of view
Explanation: 

Detailed explanation-1: -You must have the object centered before you change objectives to increase the magnification, because the field of view becomes smaller; if the object is off to the side, it may disappear when you go to higher magnification. For best viewing at high power, white light is essential.

Detailed explanation-2: -You will see more of an object on low power. The depth of focus is greatest on the lowest power objective. Each time you switch to a higher power, the depth of focus is reduced. Therefore a smaller part of the specimen is in focus at higher power.

Detailed explanation-3: -At that point – usually around 100x for standard lab microscopes – you’ll need to put a drop of oil between your specimen and the objective lens. The oil “unbends” the light to stretch out the working distance and make it possible to image at high magnifications.

Detailed explanation-4: -The specimen must be centered in the field of view on low power before going to high power because on high power, it is difficult to find the specimen.

There is 1 question to complete.