GENERAL HISTOLOGY

Detailed explanation-1: -The higher the power of magnification, the smaller the field of view. For example, if you determine that your field of view is 2.5 mm in diameter using a 10X ocular and 4X objective, you will be able to determine what the field of view will be with the high power objective by using the above formula.

Detailed explanation-2: -The light intensity decreases as magnification increases. There is a fixed amount of light per area, and when you increase the magnification of an area, you look at a smaller area.

Detailed explanation-3: -The lower the eyepiece’s magnification, the higher the field number; for example, a 10x will have a field number between 16 and 18 millimeters on average, but a 5x will be around 20 millimeters.

Detailed explanation-4: -If the total magnification increases, the diameter of the field of view decreases. The resolution limit of a compound microscope is about 0.2 microns (0.0002mm)