GENERAL HISTOLOGY

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INTRODUCTION

MICROSCOPE PARTS MAGNIFICATION RESOLUTION

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
The know field of view is 2.5 mm and an objective of 4x . What is the new field of view if you increase the objective to 40x?
A
0.25 mm
B
25 mm
C
250 mm
D
10 mm
Explanation: 

Detailed explanation-1: -Field of view is how much of your specimen or object you will be able to see through the microscope. At 40x magnification you will be able to see 5mm. At 100x magnification you will be able to see 2mm. At 400x magnification you will be able to see 0.45mm, or 450 microns.

Detailed explanation-2: -The field of view is inversely proportional to the magnification of the objective lens. For example, if the diameter of your field of view is 1.78 millimeters under 10x magnification, a 40x objective will be one-fourth as wide, or about 0.45 millimeters.

Detailed explanation-3: -When using an objective lens with a magnification of 4x, the total magnification is 40x and the field of view would have a diameter of around 4.00 mm.

Detailed explanation-4: -Field of View = Field Number (FN) รท Objective Magnification When you switch eyepieces or objective lenses, ensure to repeat the FOV calculations with the new inputs on field number and magnifications. For objects that require higher magnifications, convert your measurements from millimeters to micrometers.

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