GENERAL HISTOLOGY

Detailed explanation-1: -The electron microscopes are used to achieve up to 100, 000x magnification and more than 1000 x resolution than the light microscope. At these limits, sub-cellular structures can be easily observed. Structure of the SEM: 1-Lens : here are not the optical materials (like glass), but electrical field.

Detailed explanation-2: -TEM: magnifies 50 to  50 million times; the specimen appears flat. SEM: magnifies 5 to   500, 000 times; sharp images of surface features. STEM: magnifies 5 to  50 million times; the specimen appears flat.

Detailed explanation-3: -With electron microscopy (EM), it is possible to magnify a structure 100, 000 times. Two kinds of electron microscopic technologies have been developed: transmission electron microscopy (TEM) and scanning electron microscopy (SEM).

Detailed explanation-4: -Electron microscopes have a greater magnification because the wavelengths of electrons are much smaller than those of visible light (0.005nm as opposed to 500nm respectively–one hundred thousand times smaller). There are two types of electron microscopes, scanning and transmission.