GENERAL HISTOLOGY

Detailed explanation-1: -When using a microscope, you should always start with the lowest power objective when first viewing a specimen. Typically light microscopes have 4x, 10, 40x, and 100x objectives, therefore, you should start with the 4x objective.

Detailed explanation-2: -Place a drop of immersion oil on the top of your cover slip and another drop directly on your 100x oil objective lens. Slowly rotate your 100x oil objective lens into place and adjust the fine focus until you get a crisp and clear image.

Detailed explanation-3: -The total magnification of a low power objective lens combined with a 10x eyepiece lens is 100x magnification, giving you a closer view of the slide than a scanning objective lens without getting too close for general viewing purposes.

Detailed explanation-4: -Start by rotating the objective lens to lowest power. Place a slide on the stage, label side up, with the coverslip centered. On LOW POWER ONLY, use the coarse focus knob to get the object into focus.

Detailed explanation-5: -Start Low! Since the 4x objective lens has the least magnification, but a larger field of view, it allows for more of the specimen to be seen, as well as locating the part of the sample you wish to view. This in turn makes it easier to focus on the sample.