GENERAL HISTOLOGY

Detailed explanation-1: -The TEM offers higher resolution (  0.2 nm) than SEM, and can even reach near-atomic resolution for HR-TEM. Additionally, TEM provides information on both element and compound structure (i.e., crystal structure, elemental information and morphology).

Detailed explanation-2: -TEM users can magnify their samples by more than 50 million times, while for the SEM, this is limited to 1–2 million times. However, the maximum field of view (FOV) that SEMs can achieve is far larger than TEMs, meaning TEM users can only image a very small part of their sample.

Detailed explanation-3: -The resolution of TEM is significantly higher than light microscopes. This is because the electron has a much smaller de Broglie wavelength than visible light (wavelength of 400 700 nm).

Detailed explanation-4: -TEM uses similar principles to conventional light microscopy, but electrons are transmitted through a sample to produce an image, rather than light. This technique is a very reliable way to produce high-resolution images, which rely on the interaction of electrons with the atoms in the sample.

Detailed explanation-5: -The resolution of a TEM is about 0.2 nanometers (nm). This is the typical separation between two atoms in a solid.