GENERAL HISTOLOGY

Detailed explanation-1: -At a high power objective, the focus will change rapidly as the objective lens is adjusted, so only the fine focus knob should be used in order to prevent overshooting the correct focal plane.

Detailed explanation-2: -Fine Adjustment Knob-This knob is inside the coarse adjustment knob and is used to bring the specimen into sharp focus under low power and is used for all focusing when using high power lenses.

Detailed explanation-3: -The fine adjustment knob must only be used using a high power objective lens because the distance between the lens and the specimen slide is very short due to the larger size of the high power objective lens. The fine adjustment knob draws the lens closer to the specimen at only small distances.

Detailed explanation-4: -The fine-adjustment knob on a light microscope focuses by moving the lens slowly and is used to focus on the specimen more clearly. It is used with the high-power objective lenses. The course-adjustment knob focuses by moving the lens quickly and is used to find the specimen your are observing.