GENERAL HISTOLOGY

Detailed explanation-1: -IRIS DIAPHRAGM CONTROL-A lever (or rotating disk) that adjusts the amount of light illuminating the slide.

Detailed explanation-2: -Iris Diaphragm controls the amount of light reaching the specimen. It is located above the condenser and below the stage. Most high quality microscopes include an Abbe condenser with an iris diaphragm. Combined, they control both the focus and quantity of light applied to the specimen.

Detailed explanation-3: -Field Diaphragm-an iris diaphragm, usually located on the base of the microscope that controls the amount of light that enters the condenser diaphragm. Fine Adjustment Knob-exactly positions the specimen at the focal point of the objective lens.

Detailed explanation-4: -The iris diaphram is an adjustable shutter which allows you to adjust the amount of light passing through the condenser. The angle determines the Numerical Aperture (NA) of the condenser. This diaphragm, generally called the aperture diaphragm, is one of the most important controls on the microscope.

Detailed explanation-5: -After receiving the light from the mirror, the diaphragm directs and controls the amount of light hitting the slide. Light passes from the bottom and peaks up the image from above. This helps to make the specimen more visible.